Aequorea undefined

16 S data: The five haplotypes had a range of divergences of below 1.0 % (Table 1, intrapopulation variation). A maximum likelihood tree of the partial 16 S sequence (Fig. 37) found no significant relationship to other congeners.

Fig. 40 A-K

Observations: Small Aequorea medusae with bell diameters reaching 17 mm, only some with beginning gonad development (Fig. 40 A-B), gonad development starts at about 9 mm size. Bell in smaller animals nearly hemispherical, larger ones more flattened. Stomach small, in the larger individual diameter ~ 1 / 4 to 4 / 5 of bell diameter. Mouth rim with many broad fimbriae (Fig. 40 C). Radial canals thin, up to 46 seen, often also developing centrifugal canals. Gonads developing in some of the radial canals (Fig. 40 A), but apparently not fully developed. Up to 25 fully developed tentacles, additionally 1 - 5 very small tentacles or small bulbs without tentacles. Ratio of radial canals to tentacles 1.3 to 1.8 in individuals with visible gonads, thus always more radial canals than tentacles. Tentacles in life with a distinct, short, conical basal bulb (Fig. 40 A-B, D-E), with faint abaxial keel, emphasized or feigned by a whitish line on median of abaxial side (visible in life only), abaxial side of bulb usually continued onto exumbrella as a short abaxial spur (Fig. 40 E). Conspicuous excretory papilla on adaxial side of tentacle bulbs (Fig. 40 I), pointing into subumbrella. One to two statocysts between tentacles or small bulbs, statocysts with two statoliths.

Remarks: This species was delimited by the 16 S sequences. Were it not for the 16 S data, we would have considered the specimens as juveniles or a smaller form of A. neocyanea, this notably for their resembling tentacle bulbs (comp. Figs 38 E and 40 E). However, the 16 S sequence data (Fig. 37) separated them into two unrelated clades. The base pair differences between sequences of the two clades were> 8.2 %. The two sample sets cannot well be compared morphologically as they represent different developmental stages. None of the specimens of the present species had fully developed gonads. One difference we noted was the presence of a large excretory papilla in a majority of specimens of the present species. We were initially tempted to identify this lineage as Aequorea floridana (L. Agassiz, 1862), a species also reported to be common along the cost of Florida and the Bahamas by Mayer (1900) [for descriptions see Mayer (1910) and Bigelow (1913, 1938)]. Mature Aequorea floridana have 16 to 24 radial canals and up to 80 - 100 tentacles (Mayer, 1910). Sixteen is the prevalent number of radial canals. Our specimens with gonads had 14 to 46 radial canals and consistently fewer tentacles than radial canals. It was mainly the low tentacle number which influenced our decision to reject an identity with A. floridana. The lack of fully developed animals and the lack of distinctive features made it advisable not to introduce a new species name. This is an unsatisfactory situation, but 16 S data of fully-grown animals and also of typical A. floridana morphotypes are needed to solve the problem and to put a name on this lineage.

Material examined: BFLA 3781; 1 specimen; 18 - SEP- 2018; size 9 mm, gonads developing; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 3788; 1 specimen; 24 - SEP- 2018; no size data, no gonads; preserved in alcohol for DNA extraction; 16 S sequence MW 528637. – BFLA 3790; 1 specimen; 24 - SEP- 2018; no size data, gonads developing; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631 – BFLA 3796; 1 specimen; 13 - OCT- 2018; size 6 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 3809; 1 specimen; 19 - OCT- 2018; size 10 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 3814; 1 specimen; 20 - OCT- 2018; size 12 mm, gonads developing; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631 – BFLA 3815; 1 specimen; 20 - OCT- 2018; size 10 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 3817; 1 specimen; 20 - OCT- 2018; size 12 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 3818; 1 specimen; 24 - OCT- 2018; size 8 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 3830; 1 specimen; 15 - NOV- 2018; size 7 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence MW 528638. – BFLA 4001; 1 specimen; 20 - NOV- 2018; size 13 mm, gonads developing; preserved in alcohol for DNA extraction; 16 S sequence MW 528631. – BFLA 4002; 1 specimen; 20 - NOV- 2018; size 17 mm, gonads developing; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 4163; 1 specimen; 06 - AUG- 2019; fragments 4 and 6 mm, gonads developing; part preserved in formalin and deposited as FU- 013813, part preserved in alcohol for DNA extraction; 16 S sequence MW 528681. – BFLA 4164; 1 specimen; 06 - AUG- 2019; size 8 mm, no gonads; part preserved in formalin and deposited as FU- 013814, part preserved in alcohol for DNA extraction; 16 S sequence MW 528682. – BFLA 4167; 1 specimen; 06 - AUG- 2019; size 6 mm, no gonads; part preserved in formalin and deposited as FU- 013815, part preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528638. – BFLA 4168; 1 specimen; 06 - AUG- 2019; size 6 mm, no gonads; part preserved in formalin and deposited as FU- 013816, part preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528681. – BFLA 4205; 1 specimen; 23 - AUG- 2019; size 4 mm, gonads developing; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 4244; 1 specimen; 11 - NOV- 2019; size 10 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631 – BFLA 4443; 1 specimen; 07 - JUN- 2020; size 10 mm, no gonads; part preserved in formalin and deposited as FA- 014058, part preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631. – BFLA 4464; 1 specimen; 13 - JUN- 2020; size 9 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical MW 528681. – BFLA 4476; 1 specimen; 18 - JUN- 2020; size 5 mm, no gonads; preserved in alcohol for DNA extraction; 16 S sequence identical to MW 528631.